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Identity of [18F]exendin-4 was confirmed by HPLC comparison test with the cold standard.
Such productions could be done most conveniently and reliably by adding the desired amount of active compound to a pre-conditioned vial containing a fixed amount of cold standard.
The ELIXYS currently supports PET tracer production in our preclinical facility, and therefore, products were confirmed by coinjection with cold standard into the analytical HPLC, and radiochemical purity was found to be >99% for both tracers.
[18F]FDG produced on ELIXYS passed all quality assurance tests (e.g., radiochemical purity, pH, Kryptofix 222, pyrogenicity, sterility, residual solvent levels, TLC retardation factor (R f) comparison with cold standard, and radioisotope identity).
This was confirmed by coeluting with the cold standard of FBABM, whose NMR spectrum revealed only one isomer, on HPLC.
The 302 cold-upregulated genes found in the intersection of these lists were used to define the set of "cold standard" (COS) upregulated genes.
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Peptides DOTATATE, DOTATOC, and NODAGA-JR11 and the cold standards of their natural gallium (natGa) complexes were prepared by standard fmoc solid-phase peptide synthesis according to literature procedures (Fani et al. 2012b; Heppeler et al. 1999).
Animal procedures and monitoring were primarily done by ID with some assistance and critical input from HM. ZZ was responsible for chemical synthesis and quality control of cold standards and radiotracers.
These times agreed with those determined by spiking the radiotracer with the cold standards.
In order to address concerns that the presence of choline in the cutting solution might have altered the physiology of CINs, experiments were repeated using slices that were cut either in ice-cold standard ACSF or in a sucrose-based cutting solution consisting of (in mM): 79 NaCl, 23 NaHCO3, 68 sucrose, 12 glucose, 2.3 KCl, 1.1 NaH2PO4, 6 MgCl2, and 0.5 CaCl2.
Mice were deeply anesthetized with isoflurane and decapitated, and their brain was removed rapidly and immersed in ice-cold standard medium containing (in mM): 119 NaCl, 2.3 KCl, 1.3 MgSO4, 2.5 CaCl26.26.2 NaHCO3, 1 NandPO4 and 11 glucose, saturated with 95% O2 and 5% CO2 to maintain the pH at 7.4.
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