Sentence examples for cold stain from inspiring English sources

Use cold Stain buffer (PBS, pH 7.2, 0.5% FBS, 0.5% BSA) when preparing primary antibodies and isotypes.

Add 100 μl cold Stain Buffer/tube to resuspend cells and analyze samples immediately using BD FACSCalibur.

e. Add 200 µl cold Stain Buffer to each tube and centrifuge at 1300 rpm (240× g ) × 5 min at 4°C. f.

a. Prepare the PE-labeled human EpCAM following the manufacture's instruction for the primary and isotype staining, calculating enough total volume to add 100 µl per sample Ab stain in cold Stain buffer.

DCs were then collected and suspended in cold staining buffer (PBS containing 1% FCS, 0.1 mL) in microcentrifuge tubes.

The DCs were washed again with cold staining buffer for three times, and the cell surface markers were analyzed by flow cytometry.

Finally, cells were resuspended in 2 mL ice cold staining buffer (0.68 μM DAPI, 0.1 M Na2HPO4), filtered through a Partec CellTrics mesh (Partec, Germany) with 30 μm pore size and stored on ice until analysis.

After centrifugation at 300 ×g for 5 min at 4°C, cells were then washed 1 2 times with cold staining buffer before being subjected to flow cytometry.

Cells were first suspended and blocked in ice cold staining buffer at a concentration of 2.5×106 cells/mL for 10 min, and stained with antibodies (using antibody titration suggested by the supplier) for 30 min on ice in the dark.

The Melmet cells (∼500,000) from monolayers were resuspended in 100 µl cold staining buffer (PBS containing 0.5% FCS and 3% human immune globulin gammagard (N.V Baxter S.A, Belgium) and stained with the primary antibody, mouse anti-TRAIL-R2 (clone DJR2 4 (a.k.a.7 8), eBioscience) for 30 min at 4°C followed by staining with the secondary antibody, AlexaFluor 647-labeled goat anti-mouse (Invitrogen).

Resuspend cells and wash 2 times in cold APBS and 1 time in cold staining buffer 1. Distribute to at least 100,000 cells per stain.