Sentence examples for cold serum from inspiring English sources

incubation at 37 °C, cells were incubated at 4 °C for 1 hr., washed with ice cold PBS, fed with NDs in ice cold serum free medium, and incubated in 4 °C.

incubation, cells were incubated in 4 °C for 1 hr., washed with ice cold PBS, fed with NDs in ice cold serum free medium, and incubated in 4 °C.

A skin rash with purpura was noted after the administration of cold serum to measure cardiac output during Swan-Ganz catheter insertion.

2. Dilute the cold serum with an equal volume of cold normal saline and add one volume of cold saturated ammonium sulphate to two volumes of the diluted serum.

The cold serum box containing each serum sample was transported to the Department of Microbiology, Immunology and Microbiology in Medical faculty of the Addis Ababa University, where DAT was performed.

Matrigel (5 mg ml−1) (BD) was diluted to 1.1 mg ml−1 in cold serum free (SF) D-MEM and 100  μl of the diluted Matrigel were added into the upper chambers of the Transwell inserts (4.4  μg 100  μl−1 well) and incubated for 4 5 h at 37°C for gelling.

Three stable and one negative control cell lines were harvested by trypsinization and washed three times with cold serum-free medium.

MDA231luc cells were grown to 90%% of confluence in T-75 flasks, treated with ×1 trypsin/EDTA, washed, and suspended in cold serum-free DMEM at 80 × 106 cells per milliliter.

H69 cells (3×106) were suspended in 100 µl cold serum-free medium, mixed with 100 µl cold BD Matrigel Matrix (BD Biosciences, San Jose, CA) and 50 µM capsaicin.

The cell pellet was resuspended in cold serum-free IMDM (Iscove's modified Dulbecco's medium), and stored on ice for 10 min. Transferrin Alexa Fluor® 594 ((Molecular Probes, OR) was added at 25 µg/ml (final concentration) and cell suspension were incubated at 27°C.

Following three washing steps in ice cold, serum-free phosphate buffered saline (PBS, pH 7.5), viable tumor cells (>97%) were resuspended in PBS at a density of 1×106 cells/ml and incubated with biotinylation reagent (EZ-Link sulfo-NHS-LC-biotin, 1 mg/ml, Pierce) under gentle rotation.