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The manufacturer recommends a cold sample handling procedure to arrest ex vivo uricolysis.
These methods deliver a relatively cold sample molecules, ∼1 K, albeit with a large velocity in the laboratory frame, 100 400 m/s.
In both cryostats, the cold finger could be raised to the top of the cryostat to expose the cold sample briefly to oxygen gas and it could be heated whilst in vacuum to desorb oyxgen.
A group of 47 contigs showed elevated expression in the short term cold treatment, the prolonged cold treatment and the post cold sample (Figure 5, cluster 5).
A limited number of contigs showed a lasting response to prolonged cold; i.e. significantly changed in both the prolonged cold and 1 day after prolonged cold samples, but not the short term cold sample (60 contigs) (Figure 3).
After detachment, cells were washed in medium containing serum, centrifuged at 185 g for 3 minutes, and washed with cold sample buffer (0.1% BSA in PBS).
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In the balmy early days of winter, the wine panel, perhaps longing for some real cold, sampled 24 bottles of Amarone.
As both hot and cold samples show about the same level of scatter about the LOE.
The T test analysis on these data shows that there is no meaningful statistical difference in dynamic E between hot and cold samples within each aggregate type.
A first look at the data in Table 4 suggests that the "cold" samples have higher E values, regardless of the aggregate type.
This statistical distinction at the 95%% confidence level between top section and mid-section E values is also seen when only the "cold" samples are analyzed.
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