The respective cold diazonium salt solution was added drop wise to the mixture of coupling component with continued stirring for 2 3 h in ice bath.
The inferior turbinates collected during surgery were immediately transported to the laboratory and placed in cold physiological salt solution (PSS in mM; NaCl: 118.5, KCl: 4.8, CaCl2: 1.9, MgSO4: 1.2, NaHCO3: 25, KH2PO4: 1.2 and glucose: 10.1).
Femoral arteries were carefully dissected, immersed, and transferred to cold physiological salt solution (PSS) and then cleaned to remove the adipose and connective tissues [ 15].
Animals (either untreated or LPS-treated) were euthanized and the right common carotid artery and the right saphenous artery were isolated and rapidly transferred to cold physiological salt solution (PSS; see composition below).
The cells were resuspended in ice-cold balanced salt solution (BSS; 4 mM Na2HPO4, 2.7 mM KH2PO4, 150 mM NaCl, 2.7 mM KCl, 0.9 mM CaCl2, pH 7.2) supplemented with 0.175% bovine serum albumin (BSSA) at a cell density of 2 3×106 cells/mL.
Cortical tissue was minced in ice-cold Krebs salt solution.
Rat mesentery was dissected and placed in ice-cold physiological salt solution (PSS).
The entire mesenteric bed was removed carefully and placed in ice-cold physiological salt solution (PSS) containing (mM NaCl 119, KCl 4.7, CaCl2·H2O 2.5, MgSO4·H2O 1.17, NaH2CO3 25, KH2PO4 1.18, EDTA 0.027, and glucose 5.5, adjusted to pH 7.4.
The excised livers were washed again with chilled PBS followed by another wash with sterile ice-cold balanced salt solution (20 mM hydroxyethyl piperazine ethanesulfonic acid, NaOH [pH 7.6], 130 mM NaCl, 1 mM NaH2PO4, 4 mM glucose, and 3 mM KCl), in which the tissue was kept for 10 min.
For detection of soluble FKN in retinal tissues, mice (5 20 weeks old) were anesthetized with 5% isoflurane in an induction chamber, and following perfusion with ice-cold Hanks salt solution (HBSS, Invitrogen), eyes were enucleated, and retinas were dissected free from sclera/retinal-pigmented epithelium and disrupted manually using dounce homogenizers.
