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The resulting nanocrystals were precipitated by addition of 3 ml of ethanol to the cold reaction solution and subsequent centrifugation.
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Fifty microliters of urine was incubated with 20 μl of 2000 U/ml urease at 37°C for 60 min. Then, 420 μl of ice-cold ethanol was added into the reaction solution.
PEGylated molecules were precipitated by pouring reaction solution into excess amounts of cold ether, followed by centrifugation and then three times of washes with cold ether.
After reaction, the reactor was cooled down in cold water to quench the reaction and the reaction solution was transferred and diluted by isopropanol.
The reaction solution was precipitated overnight in cold anhydrous diethyl ether.
The reaction solution was filtered.
The reaction solution was stirred overnight.
Reaction solutions without TrxR1 were used as the control.
Reaction solutions without human Trx1 were used as the control.
Russia, it is colder reaction.
The reactor consists of three regions: the mixing and reaction area from the inlets of 1 and 2 to Y mixers connected with channels shrinking from 200 to 30 μm, the nucleation and growth area with channel width of 60 μm and length of ~30 cm, and the rapid cold quenching area with the cold quenching solution delivered at the quenching solution inlet.
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