Sentence examples for cold precipitation from inspiring English sources

The reaction was stopped with the addition of PMSF to a final concentration of 5 mM and then 300 µl of cold phosphate buffered saline (PBS) and 500 µl of cold precipitation buffer (20% w/v NaCl, 0.1% w/v N-lauroyl sarcosine in PBS) were added.

However, there is uncertainty regarding the efficiency of scavenging by cold precipitation (snow and ice).

Precipitated proteins were collected by centrifugation at 6,000× g for 10 min and washed three times with cold precipitation solution and three times with cold 80% acetone.

Curiously as early as in 1944 it was shown that SDS can be selectively removed from solutions containing proteins by cold precipitation with barium chloride [ 5].

The host component interactions that explain the higher propensity to cold precipitation in gt-1 patients support the differential regulation of HCV-Cp transcription and translation within the two major HCV gts in MCG patients.

Add 16.5 µl of ice-cold precipitation solution and mix well by pipeting up and down.

Samples were mixed with ice-cold precipitation solution (20% trichlororacetic acid in acetone) at a ratio of 1 3.

The complexes were washed three times with ice-cold precipitation buffer, and then subjected to SDS-PAGE followed by Western blotting with specific antibodies as indicated in each figure.

Our data provide evidence that HCV-Cp has a direct effect on the cold-precipitation process in a virus genotype-dependence in HCV-related MCG patients.

These data suggested that the cold-dependent insolubility of HCV-Cp was gt-dependent, implying that the dynamics of the cold-precipitation process may be different between viral gts.

By using the Architect assay to measure HCV-Cp in cryoprecipitates, we obtained evidence for a direct relation between the levels of cold-precipitated protein and the cryocrit, and thus were able to establish that the Cp concentration directly influences the amount of cold-precipitation.