Sentence examples for cold nuclei from inspiring English sources

The resulting nuclei pellet was resuspended in ice cold Nuclei EZ storage buffer, triturated, and stored at −80°C.

The supernatant, containing cytoplasmic components, was stored at −80°C, and the nuclei pellet was resuspended in ice cold Nuclei EZ lysis buffer, set on ice for 5 min, and centrifuged at 500 ×g for 5 min at 4°C.

The cells were harvested and lysed by thoroughly scraping and then transferred to a 15 ml centrifuge tube, The nuclei were collected by centrifugation at 500×g for 5 minutes at 4°C and the nuclei pellet was washed by resuspending in cold Nuclei EZ lysis buffer.

Culture dishes were placed on ice and 4 ml of ice cold Nuclei EZ lysis buffer (Nuclei Isolation Kit from Sigma, Deisenhofen, Germany) were added.

Briefly, the cells grown in tissue culture Ø 10 cm dish, washed with ice cold PBS twice and then 4 ml of ice cold Nuclei EZ lysis buffer was added to each dish.

Cells were washed twice with cold PBS and subsequently incubated on ice for 10 min with cold nuclei lysis buffer (50 mM Tris HCl, 10 mM EDTA, 1% SDS) to which 1 mM protease inhibitors (Sigmafast, Sigma Aldrich, St Louis, MO) and 4 mM PMSF (Sigma Aldrich, St Louis, MO) was freshly added.

Nuclei were gently resuspended in ice-cold Nuclei Resuspension Buffer/MNase Digestion Buffer (NRBT/MDB: 50 mM TRIS-HCl pH 7.5, 320 mM sucrose, 4 mM MgCl2, 1 mMCaCl2, 1% Triton, 0.1 mM PMSF), flash frozen, and stored until use.

Cells were washed in ice-cold nucleus buffer (0.15  M NaCl, 5 m M MgCl2, 1 m M KH2PO4, 1 m M EGTA, 0.1 m M Dithiothreitol, 10% glycerol in distilled water pH 6.5).

After 15 min tumbling in the cold room, nuclei were pelleted (10,000 g, 10 min) and supernatants were fractionated over 10 50% linear sucrose gradients using ultracentrifugation (35,000 rpm, 3 h 10 min) in a Beckman ultracentrifuge and an SW40 Ti rotor.

Nuclei were then resuspended in ice-cold nuclei lysis buffer (50 mM Tris HCl pH 8.0, 10 mM EDTA, 0.1 mM phenylmethanesulfonylfluoride (PMSF) 1% SDS).

Ice-cold nuclei EZ lysis buffer (2 ml) was added, and the cells were scraped with a small bladed cell scraper.