Sentence examples for cold lysis solution from inspiring English sources

Then the slide was immersed in cold lysis solution to lyse the cells.

Slides were incubated in cold lysis solution (Trevigen) for 45 minutes at 4°C.

Cells were washed with PBS and then lysed in cold lysis solution (5 mmol/L of Tris, pH 7.4, 20 mmol/L of EDTA, 0.5% Triton X-100) for 20 min [16].

Beads were collected by centrifugation and washed twice for 10 min at 4°C in cold lysis solution prior to a final wash in cold PBS.

These slides were immersed in freshly prepared, cold lysis solution added with 1% triton X-100 at 4°C for 1 hr in a dark place.

For RNA extraction, 25000 SP and 25000 MP cells were sorted by FACS into cold lysis solution (RNeasy Micro Kit; Qiagen, Venlo, The Netherlands).

Treated and untreated cells were lysed into ice-cold lysis solution containing RIPA buffer, as previously described [28].

These agarose sample plugs were immersed in ice-cold lysis solution (0.5  M EDTA; 0.01  M Tris; 2% Sarcosyl; 0.2 mg ml−1 proteinase K) for 1 h and then incubated at 50°C for 48 h.

Plugs containing cells were extruded into 1 ml of ice-cold lysis solution (0.4  M EDTA, 2% sodium N-lauryl sarcosine, 1 mg ml−1 proteinase K, pH 8.0) in Eppendorf tubes for 30 min.

We have increased the sensitivity of the assay by embedding the cells in agarose prior to irradiation at 0°C; slides can then be placed in ice-cold lysis solution immediately following irradiation.

Slides were then incubated in ice-cold lysis solution [2.5 M NaCl, 10 mM Tris, 100 mM EDTA, 1% Triton X-100 and 10% DMSO, pH 10.0] at 4°C for at least 1 h.