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After cold loading, cells were further incubated for 5 h at 37°C in DMEM without serum.
The pellets were resuspended in 25 mL of cold loading buffer (0.15 M NaCl, 25 mM Tris HCl, pH 7.8, 25 mM CaCl2) and lysed by sonication.
Similar(58)
Their search required a helium cold load that made their experiment sensitive to the CMB.
In order to make the radiometer system as simple as possible, we use the sky as the calibration cold load.
We will use gas gap heat switches to alternately connect the toroid to the cold load and the warm heat sink.
Here we show the importance of those factors as well as their detrimental effect in heterogeneity using cold loads as a case study.
This study is useful especially for frequently energized distribution transformers having insignificant protection against cold load pick up and inrush current.
We anticipate similar realistic models to be used for real world applications and aggregation methods based on them, especially for cold loads and similar TCLs, where external factors and heterogeneity in time are significant.
The heat and cold load can be supplied by MTs.
Cold load pickup is serious concern in load restoration without enough research yet.
To evaluate load restoration amount, the cold load pick-up (CLPU) characteristics should be considered [58].
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