Sentence examples for cold fractionation from inspiring English sources

Cells were incubated for 5 min in 900 μl of cold fractionation buffer (20 mM Tris HCl [pH 8.0], 150 mM NaCl, 10 mM NaF, 1 mM DTT, 250 mM sucrose) containing EDTA-free protease inhibitor cocktail (Roche) and phosphatase inhibitor cocktails (set II+IV, Calbiochem).

Human intravenous immunoglobulins prepared by the cold ethanol fractionation technique of Cohn are considered safe with respect to infectivity.

The principal manufacturing process in all current Ig preparations is cold ethanol fractionation with product-specific additional processes for manufacturing.

The first commercially available preparations of intravenous human albumin solution were developed using the cold alcohol fractionation technique created by Edwin Joseph Cohn [ 9, 11].

Cycloheximide was added to a final concentration of 0.1 mg/ml, and cells were harvested (4000 rpm, 4 min, 4°C) and resuspended in 400 µl of ice-cold membrane-fractionation buffer (20 mM Hepes-KOH pH 7.6, 100 mM potassium acetate, 5 mM magnesium acetate, 2 mM dithiothreitol, 0.1 mg/ml cycloheximide and 0.1 mM PMSF) either with or without additional 20 mM EDTA.

Briefly, the cell pellet was resuspended in ice-cold PARIS cell fractionation buffer and incubated on ice for 10 min to lyse the cells.

BrdU labeling, fixation in cold 70% ethanol, cell cycle fractionation by flow cytometry and isolation of BrdU-labeled DNA by immunoprecipitation were carried out as previously described [22].

A second cell pellet obtained at the same time was homogenized in ice-cold cell disruption buffer without fractionation to give a whole cell lysate.

The use of surfactants and detergent fractionation to improve the cold flow properties of biodiesel from waste cooking oil (BWCO) was investigated.

Neuroimaging studies suggest that the RCT and WCST may recruit partially dissociable neural circuits (21,22), consistent with this fractionation of hot and cold executive function.

A whole mouse heart from mice were excised and washed in cold PBS and the cytosolic and mitochondria fractions were derived following the Mitochondrial/Cytosol Fractionation Kit manufacturer's protocol (DBI Bioscience).