Sentence examples for cold fixation from inspiring English sources

In the silence, emptiness echoes, too: the cold fixation of compulsive behaviour.

Adult mice under deep anesthesia were transcardially perfused with cold PBS followed by cold fixation buffer containing 4% paraformaldehyde in PBS.

After repeated cold fixation for 15 min, the cells were used for banding and cytogenetic analyses.

Consequently, we developed a new cold fixation protocol (70% ethanol fixation at -10°C), allowing efficient single-cell capture.

For immunogold labelling, the samples were prepared by a minimal, cold fixation and embedding in Unicryl resin (British BioCell International, Cardiff, UK) procedure, as we have previously described in detail (50, 51).

SH-SY5Y cells and brain tissues were prepared for immunogold labeling TEM by minimal, cold fixation and embedding protocols, as previously described in Soura et al., (2012) [ 33] and Thorpe et al., (2001) [ 34], respectively.

Prefixed cells were centrifuged and resuspended in ice-cold fixation solution (2 % paraformaldehyde and 2.5 % glutaraldehyde) overnight.

For quantification of ERK and pERK levels, 50 ventral ganglia from late third instar larvae were dissected in ice-cold fixation solution (4% paraformaldehyde in PBS) to minimize changes in phosphorylation status during handling, fixed for 10 min on ice and washed three times for 20 min in PBS.

Typical specific immounolabelling was present in the more conventional cold formalin fixation followed by Triton permeabilization, but the data were replicated also with the other fixation methods that were tried, albeit with different final intensities of the labelling, or with different background.

Is it because the NATO boss, America, has the cold war fixation of using Turkey against Russia?

Immunohistochemistry for CD11b (Pharmingen, dilution 1∶100), CD45 (DAKO, dilution 1∶100) and KDR (Santa Cruz, dilution 1∶500) were performed on 5 µm frozen sections according to standard procedures using 5 min cold acetone fixation and 1 hour incubation with the primary antibody.