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All the aromatic niceties remain intact without the fatty acids and esters that are removed by cold filtering.
When whisky is diluted with water to 40% alcohol these oils can give the whisky a cloudy appearance, and so for improved shelf appearance they are generally removed by cold filtering.
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"You precariously take that glass sphere, fill it with cold filtered water, then you stand on a stepstool and nervously and quickly invert it over the reservoir," said James Freeman, the owner, explaining the process.
But instead of just pitching bottled water to the sweaty masses, Bonnaroo has installed giant "hydration stations" around the venue where concert goers can refill their reusable containers with cold filtered water for free.
The gonads containing numerous oocytes were dissected from the central dorsal area and transferred to cold filter-sterilized seawater.
Free oocytes were isolated as single cells by sieving through gauze several times followed by repeated rinsing and low speed (<1,000 rpm) sedimentation in cold filtered seawater.
The isolated tissue was transferred to a dish containing cold filtered 1xPBS and then cut into small pieces.
At the surface, the bones were stored in cold filtered seawater (4°C) before removal of the embedded Osedax worms.
Matrigel (Collaborative Biomedical Products, Bedford, MA) was diluted to 0.5 mg/ml with cold filtered distilled water and applied to 8-mm pore size polycarbonate membrane filters.
Ice and cold filtered water.
Then 1 ml of ice-cold, filtered 77% ethanol was added for fixation.
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