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The cold factor is rather stable at a large variety of operation conditions, i.e. climate conditions.
The maximum so-called cold factor for the SolabCool® is 0.55.
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They can modulate the interaction of defense pathways activated by biotic (pathogen infection) and abiotic (wounding, drought, salt, and cold) factors [ 68].
Moreover, a previous study showed an APETALA2 gene subclade IIIc (COLD BIND FACTOR II gene) is involved in regulation of apple pulp softening during cold storage and/or ethylene treatment [ 22].
The cold instrument factor is another disincentive.
The cold sensitivity factor is defined in Figure legend 1 as the ratio of growth at 37°C to growth at 15°C, normalized to the mean of all cold sensitivity factors calculated.
1) Presumably the cold sensitivity factor is the ratio of growth at 37°C, relative to 15°C, relative to wild type?
Each strain's cold sensitivity factor is defined as the ratio of growth at 37°C to growth at 15°C.
A cold sensitivity factor was subsequently generated for each clone, defined as the ratio of growth at 37°C to growth at 15°C, normalized to the mean growth ratio measured for the entire collection.
Strains that displayed a cold sensitivity factor in the top 3.5% (155 clones) were analyzed using clusters of orthologous groups (Tatusov et al., 1997, 2003) to categorize the cellular function of each deleted gene.
For tumor growth experiments on the CAM, 5 × 10 HT29 cells or 7.5 × 10 OE19 cells were resuspended in 50 μL cold growth factor reduced Matrigel (Becton Dickinson, Breda, The Netherlands) and kept on ice until grafting.
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