Sentence examples for cold extraction from inspiring English sources

The grounded powder (500 g) was macerated with methanol (2.5 L) and extracted by cold extraction process.

The cold extraction was used to extract the oil from durian seed flour in order to avoid the thermal degradation.

The natural sensitizers are extracted using a cold extraction and the Soxhlet extraction methods.

The plant parts were extracted by a cold extraction method.

Dried powdered root (500 g) of V. roxburghii was extracted exhaustively with methanol by cold extraction method.

The powder obtained was extracted via the method of cold extraction using ethanol and then kept for a period of 5 days accompanying occasional shaking and stirring.

Whole-cell protein extracts were prepared by trypsinizing cells and lysing in ice-cold extraction buffer (10 mM Imidazole, 100 mM NaCl, 1 mM MgCl2, 5 mM Na2EDTA and 1% Triton X-100) and homogenizing the cells using a pipette tip.

Briefly, each leaf was homogenized in liquid nitrogen and ice-cold extraction buffer (50 mM sodium phosphate buffer, pH 6.8, and 1 mM hydroxylamine) then added.

The cells were harvested by centrifugation at 5,000× g for 10 min, washed with 50 mL of ice-cold extraction buffer (100 mM sodium phosphate buffer (pH 7.8), 0.15 M glycerol, 1 mM DTE, 1mM PMSF, 1 mM EDTA).

The nuclear pellet was resuspended in ice-cold extraction buffer- 20 mM HEPES, pH 7.9; 0.4 M NaCl; 1 mM EDTA; 1 mM EGTA; 1 mM DTT; 1 mM PMSF and 2 µg/ml each of aprotinin, leupeptin and pepstatin.

Treated astrocytes were harvested and suspended in 0.5 ml of ice-cold extraction buffer (25 mM Tris pH 7.4, 25 mM glycine and 150 mM NaCl) containing 1% Triton X-100, 1× complete protease inhibitor cocktail and 5 mM EDTA.