The powder (50 g) was cold extracted in water with intermittent shaking to mimic the traditional local method of extraction and later lyophilized to obtain a dry powder.
Raw, cold extracted honey hasn't been heat-treated and "purified", so still contains its full complement of enzymes and antioxidants, and has antibacterial properties.
This study highlighted the HHP as a promising technology to cold extract phenolic compounds (phenolic acids and flavonoids) from watercress in a selective way using a green solvent and reduced extraction times.
If you have to use a sweetener, why not use cold extracted honey, which is as natural as they come?
For crude nuclear extracts, the cell pellet of 1.5×107 monocytes was resuspended in 400 µl ice cold extract buffer (20 mM HEPES, pH 7.9, 0.1 mM EDTA, 0.1 mM EGTA, 1 mM DTT) containing 10 mM KCl, placed on ice for 20 min and lysed in 0.5% Nonidet P-40.
After centrifugation, the pellet was resuspended in 50 µl ice cold extract buffer containing 20% glycerol and 400 mM NaCl and incubated at 4°C for 30 min. After centrifugation, crude nuclear extracts were microdialyzed against PBS, used immediately or stored at −80°C.
Powdered husk of Cocos nucifera was cold extracted using mixture of methanol and distilled water in ration 3 2 (v/v).
The chemical study of As-HAE allowed identifying the flavonoids found in cold extract: luteolin, quercetin, and 3- O-methylquercetin, but at higher concentrations.
However, As-HAE as well as cold extract did not induce apoptosis measured by Hoechst 33258 staining, TUNEL assay, and DNA fragmentation analysis.
The in vivo micronucleus test showed that As-HAE exerted cytogenotoxic effects on bone marrow of mice, contrary to what was observed with cold extract.
