Sentence examples for cold collagen solution from inspiring English sources

mFBs were harvested from mono-culture, counted and resuspended in pre-mixed cold collagen solution at a density of 1 × 106 cells/mL.

The collagen lattice was prepared by combining 20 μL of cold collagen solution (BD, USA), 80 μL co-culture medium and 0.46 μL of 0.1 mol/L sodium hydroxide solution on ice.

Cold collagen solution (0.3 ml) was poured into 8-wells chamber wells (Nunc) avoiding bubble formation and warmed to 37°C to induce polymerization.

The ice cold collagen solution (80% of total volume, 4 mg/ml) was mixed with Hanks salt 10x with phenol red (10% of total volume) and adjusted to pH 7.4 by adding about 80 µl of 2 M NaOH per 12 ml collagen mixture while gently stirring on ice to avoid air bubbles and premature gelation.

Type I collagen gel was prepared using an 8 1 1 ratio of cold collagen solution (Cellmatrix I-P; Nitta Gelatin, Osaka, Japan), 10 × concentrated MEM (Invitrogen), and collagen dilution buffer containing 0.05 N NaOH, 2.2% NaHCO3, and 200 mM HEPES, pH 7.4.

Ice cold collagen solution (80% of total volume, 4 mg/ml) is mixed with 10x Hanks buffer (10% of total volume) and adjusted to pH 7.4 by adding 80 µl 2N NaOH per 12 ml while gently stirring on ice (i.e. for 6 gels 12 ml collagen + 1.5 ml 10x Hanks buffer).

E13.5 neurospheres were dissociated and suspended in ice-cold collagen solution at a density of 5 × 10 cells/ml.

A total of 500 μl of ice-cold collagen solution (16 ml of Vitrogen 100 purified collagen type I (Collagen Corp., Palo Alto, CA, USA), 2 ml 10 × Dulbecco's modified Eagle medium (DMEM) and 2 ml of 0.2 M 4- 2-hydroxyethyl -1-piperazineethanesulfonic acid (HEPES)) was added to each bovine serum albumin (BSA)-treated well and incubated for 1 h to allow gelation.

11 Briefly, 1.0 × 10/mm fibroblasts were mixed with a cold collagen gel solution, then 0.5 mL fibroblast collagen mixture was added per well in a 24-well plate, and incubated for 1 h at 37°C.

After two hours, the medium was aspirated gently and unless otherwise stated, 0.2 ml of the same cold neutralized collagen solution was added to cover the cells and to form the upper layer of collagen.

Cover glasses were treated with 1 mg/ml acidic collagen solution (KOKEN) for 30 min at room temperature, washed twice with ice-cold water, and dried on a clean bench.