Sentence examples for cold buffer solution from inspiring English sources

Embryonic cortical brains were dissected in ice cold buffer solution under a microscope (Leica M651, Buffalo Grove, IL, USA).

During all steps the gut is often rinsed with the ice cold buffer solution in order to prevent drying.

Routinely, the harvested cell pellet was lysed using a cold buffer solution containing 50 mM Tris, 0.5 mM EDTA, 0.5 M NaCl, 0.04% NaN3, and 1% IGEPAL (pH 7.4).

The lungs were immersed in cold buffer solution (composition, see below) and the bronchi were freed of adhering lung tissue down to the third generation (0.5 mm) by dissection under a microscope.

Biopsies were immediately stored in ice-cold buffer solution (Dulbecco's phosphate buffered saline and indomethacin, final concentration 10 μM) and mounted on adequate tissue sliders.

The aorta was then cannulated under the buffer surface with a 21-gauge tailor-made cannula constructed from a blunted needle attached to small length of plastic tubing that had been prefilled with ice-cold buffer solution to prevent air bubbles from entering the system.

Five millimeter fragments of spinal cord tissue centered on the injury site were collected from rats at day 1 after injury and homogenized in ice-cold buffered solution containing 0.32 M sucrose, 10 mM Hepes and protease and phosphatase inhibitors.

Briefly, astrocytes were placed in 24-well for 12 h, then treated with 4, 8, or 12 μg/ml of ZnO NPs for 6, 12, or 24 h, incubated with DCF diacetate in culture medium for 15 min, and washed with cold phosphate buffer solution three times.

The resident peritoneal macrophages were obtained by injection of cold phosphate buffer solution (PBS) 1x (5 mL, 3x) into euthanized mice abdominal cavity.

At each identified concentration of TH and at different incubation times, 1 × 10 cells from culture medium were isolated, washed with ice cold phosphate buffer solution, resuspended in 100  μL binding buffer, and stained with 5  μL of FITC-conjugated Annexin V (10 mg/mL) and 10  μL of PI (50 mg/mL).

Slides were then immersed in cold lysis buffer solution [2.5 M sodium chloride (NaCl), 100 mM EDTA, 10 mM Tris (hydroxymethyl) aminomethane hydrochloride, 10% dimethylsulfoxide (DMSO), and 1% Triton X-100, pH 10.0] for 1 hr to dissolve the membranes and break down the protein matrices.