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In vitro autoradiography experiments were carried on rat brain slices by incubating tracer and cold blocking solution.
Jessie Carrier stood for hours in the cold blocking one of the side entrances.
Permeabilisation buffer was replaced with 1 mL of cold blocking buffer (0.1 % Triton-X, 0.2 % skimmed dry milk in PBS) at 4 °C overnight.
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The samples were incubated for 30 min on ice before addition of 140 μL of ice-cold blocking solution (20 mg/mL BSA and 2 mg/mL yeast mannan) and 1.4 mL of an ice-cold chloroform/methanol mixture (1 1, v/v) to produce a final chloroform:methanol:water ratio of 10 10 3 (v/v/v).
Cells were stimulated with LPS (10 ng/mL) or fMLP (10−13 M) for 48 hr, dislodged in HBSS, pelleted (1,000 × g for 5 min at 4°C), and resuspended in ice-cold blocking solution (HBSS containing 1% BSA) for 20 min. We washed cells and stained them for 30 min on ice with a 1 200 dilution of phycoerythrin-conjugated anti-rat OX6 (BD Pharmingen, San Diego, CA).
It is the second time this year that the ICO has fined a company for cold calling people about cold call blocking equipment.
Samples were kept on ice before and after sorting and a cold block was used to hold sample tubes during the sort.
This "cold block" signifies competition for a receptor, possibly a LRP receptor [52].
This "cold block" signifies competition for a receptor to the point of saturation.
Seeding was performed at the tip of the test tube as the samples entered the cold block (at −10°C).
The samples were then flash-frozen in 96-well cold block (−80°C) for 15 s and then immediately thawed in a 96-well heat block (96°C) for 15 s.
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CEO of Professional Science Editing for Scientists @ prosciediting.com