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This "cold block" signifies competition for a receptor, possibly a LRP receptor [52].
This "cold block" signifies competition for a receptor to the point of saturation.
Seeding was performed at the tip of the test tube as the samples entered the cold block (at −10°C).
The samples were then flash-frozen in 96-well cold block (−80°C) for 15 s and then immediately thawed in a 96-well heat block (96°C) for 15 s.
250 µl aliquot of stored sample was mixed with 1 ml of acetonitrile/0.4% acetic acid at −25°C and vortexed for 45 60 sec, then temperature was brought to −15°C in a cold block, and vortexed again for 30 45 sec.
The final reconstituted samples were incubated in a cold block (∼4 °C) for 1 h before crystallization.
Similar(49)
In vitro autoradiography experiments were carried on rat brain slices by incubating tracer and cold blocking solution.
Jessie Carrier stood for hours in the cold blocking one of the side entrances.
Permeabilisation buffer was replaced with 1 mL of cold blocking buffer (0.1 % Triton-X, 0.2 % skimmed dry milk in PBS) at 4 °C overnight.
The tube was vigorously vortexed for 30 s and then cooled for 1 min at 4°C in a cold-block.
Resolved proteins were transferred to Hybond-P PVDF membranes or onto Immobilon-FL in 0.1 M Tris-base, pH 8.3, 0.192 M glycine and 10% (v/v) methanol using an electrophoretic transfer system with cold-block.
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