Sentence examples for cold assay from inspiring English sources

The collected proteoliposomes were washed twice with the cold assay buffer.

The proteoliposomes were on ice and resuspended in cold assay buffer before use with a final concentration of 100 mg/mL.

3) The authors should characterize K-fiber stability (even if by just a crude cold assay) after TACC3 overexpression.

Mouse monoclonal antibodies were diluted in cold assay buffer (PBS, pH 7.2, supplemented with 0.1% FCS) and added in a 50-μl volume.

In short, yeast strains expressing gat-1 fused to GFP were grown to an OD (600 nm) of 4.0 to 5.0 in selective media, washed 3 × with ice cold assay buffer (30 mM MES-NaOH (pH 5.6) and 50 mM ethanol), and resuspended to an OD of 40.0.

For the scaled up cold assay, a 60 µl reaction included: 100 m m Tris HCl pH 8.3, 1 m m DTT, 2 µ m SAM, 50 n m PRC2 and 0.1 µ m nucleosomal array, with or without 40 n m FLAG-huntingtin (4 h, 30°C).

There was also no difference in responses between WT and Entpd3 -/- mice in any of the cold assays (acetone evaporative cooling, cold tail immersion at -10°C, or cold plantar; Table 3).

In radioactive assays, 20 μM ATP and 2 μCi [γ−32P] ATP (Amersham, 370 MBq ml−1, 3000 Ci mmol−1) were added, or 1 mM ATP was used in cold assays.

Bound and free radioligand were separated by vacuum filtration through glass fiber 96-well filter plates (Millipore Multiscreen FB filter plate), followed by washes using ice-cold assay buffer (3 × 150 μL).

After incubation for additional 2 hour, the immunecomplex was washed three times in the ice-cold assay buffer at 4°C before SDS-PAGE.

In brief, cells were lysed in ice-cold assay buffer.