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Leave to cool in the tin, but remove from the tin before fully cold and transfer to a wire rack to cool completely.
Remove supernatant, re-suspend the cells in 5mls of fresh growth medium (warm or cold), and transfer to a 25cm flask.
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Drain immediately, rinse with cold water and transfer to the bowl with the dressing.
5 Flick the choux with a little cold water and transfer to the oven (which should still be at 180C) for 13 minutes.
Wash the tissue grinder and pestle with 2×100 μl of ice-cold methanol and transfer the washes into the sample vial always using a glass Pasteur pipette.
For eCBs extraction, heart tissues were first weighted in the cold room and transferred to precooled 2 mL Precellys tubes containing cold ceramic beads.
We propagated the majority of species in individual seedling cells (3.8 × 3.8 × 6 cm) in summer 2010, held overwinter in a cold-room or cold-frame, and transferred them to a warm environment on 12 January 2011.
Equal amounts of E. coli purified proteins were run on SDS-PAGE after in vitro kinase assay (with cold ATP) and transferred onto nitrocellulose membrane (Bio-Rad).
The pellet was washed 1x with 25 mL of ice cold water and transferred to a 50 mL falcon tubes.
The resulting pellets were washed with cold PBS and transferred to 1.5-ml eppendorf tubes and centrifuged 5 min at 2000 rpm.
Blood samples were placed in ice cold water and transferred to the central laboratory to be analyzed by co-oximetry (ABL 725; Radiometer, Copenhagen, Denmark).
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