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He was taken to St . Lukes-Roosevelt Hospital Center, feeling cold and dehydrated.
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There are some differences between the absolute gene expression levels of the cold and salt dehydrated animals.
Tissue samples were fixed in cold 4% paraformaldehyde, washed with cold phosphate buffered saline and dehydrated in 30% sucrose.
Seventy-two hours after lentiviral PICT1-shRNA infection, cells were fixed in 4% formaldehyde and dehydrated with cold methanol-acetone (1 : 1).
Slides were subsequently denatured in 75%% formamide (pH 7.0) (Sigma-Aldrich) in 2× SSC at 74 °C for 3 min, and then immediately cooled and dehydrated in 70%% (cold), 80 % and 96%% (RT) ethanol.
The following day, after washing, the tissue was stained with a solution of lead aspartate for 30 min at 60 °C (pH 5.5), washed again and dehydrated using ice-cold solutions of freshly prepared 35, 50, 75, 80, 90, 95 and 100%% ethanol.
The slides were denatured in 70% formamide/2xSSC, pH 7.0 for 40 s, and dehydrated in an ice-cold ethanol series.
Slides were rinsed twice in 2X SSC and dehydrated with 70 %, 90 % and 100changes of cold ethanol at −20°C for 5 min each.
The 6-mm block specimens were fixed and dehydrated in graded ethanol (70 100%) prior to cold embedding in poly methylmethacrylate) (Erben 1997).
Slides were washed in 2× SSC at 37°C for 30 minutes, and dehydrated sequentially in 70%, 80%, and 95% ice-cold ethanol.
We were hot and dehydrated.
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