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Mononuclear cells were separated on Ficoll-Hypaque and enriched for monocytes using cold aggregation [55].
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A similar aggregating species can also be formed in the absence of clusterin by employing cold-induced aggregation [ 9].
Figure 2 shows representative cold-induced aggregation curves obtained after performing the rapid screening test on type I and type II cryoglobulinemic serum samples and on a control serum sample normalized to the type II curve profile; moreover, parameters derived from the phenomenological analysis of the process are reported.
I. Cold-induced platelet aggregation.
For in vitro experiments hemolymph from 12 animals were diluted 1 1 in ice-cold filtered sea water to prevent aggregation and dispensed (200 μl) into 24 wells plates.
The molecules of PEG cover gold nanoclusters just after ablation and act like "bumpers", limiting contact between particles, preventing their coalescence (when the particles are still "hot") and aggregation (when the particles are "cold").
The multicoloured Asian ladybirds, Harmonia axyridis (Pallas), form large aggregations inside dwellings to survive cold winters, causing annoyance to householders due to their abundance, allergenic properties and problems consequent on reflex bleeding.
Carbon nanoparticles suspended in cold distilled water have fewer interactions, so less aggregation occurs, resulting in smaller nanoparticles.
The cold hard reality is that based on the aggregation of current international commitments on greenhouse gas reduction, the planet will not be able to sustain temperature increases within the 2 degree threshold.
We anticipate similar realistic models to be used for real world applications and aggregation methods based on them, especially for cold loads and similar TCLs, where external factors and heterogeneity in time are significant.
Wheat WZY2 can act as a protectant to increase Escherichia coli viability, protect lactate dehydrogenase (LDH) activity and inhibit protein aggregation during temperature variation including heat and cold (Yang et al. 2015).
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