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Ovigerous Lepeophtheirus salmonis were collected from adult Atlantic salmon following harvest from a farm near Vancouver Island and transported in ice cold aerated seawater to Nanaimo.
The brain was dissected out and placed in ice cold aerated (95%% O2, 5%% CO2) sucrose artificial cerebrospinal fluid containing (aCSF, in mM) sucrose (217), NaCHO3 (26), KCl (3), MgSO4 (2), NaH2PO4 (2.5), CaCl2 (2) and glucose (10).
Subsurface waters are diluted with cold, aerated surface water.
The tissue was continuously perfused with cold aerated Ringer's solution during the entire incubation period.
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Liver cores were placed in a Krumdieck tissue slicer (Alabama Research and Development, Munford, AL) filled with ice-cold KHB aerated with carbogen and supplemented with 11 mM glucose.
Liver cores were placed in a Krumdieck tissue slicer (Alabama Research and Development, Munford, AL, USA) filled with ice-cold KHB aerated with carbogen and supplemented with 11 mM glucose.
The MMA was removed from the dura mater by a dissecting microscope, cut into segments 1 2 mm long that were placed in parallel tissue baths of ice-cold bicarbonate buffer solution aerated with a gas composed of 95% O2 and 5% CO2, with a resulting pH of 7.4 [23].
It is aerated with all sentient beings, so aerated that there's no solidity at all.
Brains were incubated in ice-cold slice solution (in mM: 26 NaHCO3, 1.25 NaH2PO4, 10 glucose, 125 NaCl, 3 KCl, 5 MgCl2, 1 Caeratederated with 95%O2/5%% CO2 for 5 min, and 300 µm coronal sections were collected using a vibratome and further microdissected in HBSS under a 10X dissecting microscope.
When aerated, it unfolds beautifully.
Calm down, dear, don't get aerated.
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