Sentence examples for cold acid from inspiring English sources

Receptor bound (acid releasable) radioactivity was then removed using 2 × 250 μL of ice cold acid wash buffer (0.02 M NaOAc buffered with AcOH to pH = 5).

After DNase treatment and additional cold acid phenol chloroform extractions, a final chloroform:isoamyl alcohol extraction was performed.

The cells were then placed on ice, washed with cold PBS containing 0.2% BSA, 1 mM CaCl2 and 1 mM MgCl2, incubated with cold acid buffer (0.2 M acetic acid, 0.5 M NaCl) and ice-cold PBS, fixed, and mounted in Vectashield with DAPI Vector Laboratoriess, Burlingame, CA).

Cells were then washed twice in cold PBS and twice in cold acid buffer containing 0.2 M acetic acid + 0.5 M NaCl, pH 2.8, followed by two more washes in cold PBS before fixation in 4% PFA and DAPI staining.

Non-internalized biotinylated CTB was masked by successive treatment with 50 µg/ml of avidin (Sigma-Aldrich) for 1 hr on ice, followed by three 1-min cold acid washes (0.2 M acetic acid/0.2 M NaCl).

We recently developed proteomic and transcriptomic tools to monitor in vitro gene expression under different stress conditions including heat, cold, acid, bile salts or conditions encountered within cheese curd [ 21, 23, 24, 28- 30].

The resulting powder was then suspended in cold acid-ethanol, and insulin was extracted overnight at 4°C [32].

Uptake was stopped by washing twice with ice-cold acid wash (150 mM NaCl, 20 mM citric acid, pH 5.0).

The acid eluates from the dishes were collected and each dish received another 1 ml of ice-cold acid buffer to wash the cells.

Pancreatic insulin content For detection of total pancreas insulin, whole pancreases were homogenised in ice-cold acid ethanol (0.1 mol/l HCl in 70% ethanol) and incubated 24 h at 4°C.

To determine the surface-bound radiolabelled peptide, ice-cold acid buffer (0.1  M acetic acid, 154 m M NaCl, pH 2.5) was added and cells were incubated for 10 min at 4°C.