Sentence examples for cold 1 from inspiring English sources

For oocytes, the cells were fixed by addition of ice cold 1% formaldehyde in methanol (1 ml/5–10 oocytes) and incubated at −20°C for 2 hrs.

The cells were pelleted and re-suspended in cold 1 × binding buffer.

L3 larvae, pupae and adult brains were dissected in cold 1 × PBS.

Mice were decapitated and the cochlea was dissected from the skull in cold 1 × PBS.

For sequencing, frozen library aliquots were scraped and placed into 1 ml ice cold 1 × PBS.

Ten million cells were washed twice with ice cold 1 × PBS, gently scraped and collected by centrifugation.

Previous analyses already demonstrated that gene expression becomes arrhythmic under L/L in the cold [1].

At the end of incubation, cells were washed twice with cold 1× HBSS buffer.

Pellets were resuspended in 100 µl cold 1× PBS on ice.

Media was aspirated and cells were then washed with 1 ml of cold 1× PBS and the PBS aspirated.

Salivary glands of third instar larvae were hand dissected in ice cold 1× PBT (PBS+0.1% Tween).