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Tilling was performed in a Col line that carries the fast-neutron-induced er-105 mutation [16].
Out of the total 27,004 genes in the Arabidopsis thaliana Columbia (Col) line, 2741 genes (10%) were found absent in one or more genomes of the 80 Arabidopsis accessions using our approach.
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However, the HPL-OE lines contain significantly lower levels of camalexin (∼30%) than that in Col lines (Figure 4D).
This reduction is potentially attributable to the ∼60% decrease in the JAs levels in HPL-OE as compared to Col lines (Figure 1B &C).
(A ) Western detection of RBR-Myc in transgenic plants harboring the FAMAp RBR-MYC construct in either Col (lines 1–4) or FAMAp RBR-MYCs 1–3) baconstruct.
We examined the cotyledon-opening angles by calculating the medians split on significant parent markers for Ler x Cvi and Ler x Col lines.
Figure 1 r d dependence of t col (solid lines) and t spin-down (dashed lines) at R 1 = 1.
Arabidopsis Col-5 line is a glabrous derivative of Col-0.
Also, there is a possibility that there were DNA sequence specific differences between the A. thaliana FN1148 Col-0 mutant (obtained from J. Schroeder's lab, UC San Diego, USA) and the control A. thaliana Col-0 line that had been propagated in our own laboratory since the 1990's.
Chloroplast and starch granule morphology of Atidd5 and col mutant lines were examined by transmitted electron microscopy (TEM).
Transmission electron micrographs of Atidd5 and col mutant lines and wild type are shown in Figure 7.
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