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Collectively, these data provide a compelling argument in favor of the candidate CRMs being bona fide regulatory elements in their own species, and not merely emulating Drosophila CRMs by virtue of coincidental sequence similarities.
An increase in marker density for these regions may provide further evidence as to whether these results are indicative of true deletions or coincidental sequence divergences at the primer site(s).
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Although potentially coincidental, the mature sequence for miR-183 in vertebrates is very similar to that of both D. melanogaster dme-miR-263a and miR-263b (Figs. 4B and 4C).
This overlap of pan-WNV sequences with non-viral sequences is possibly coincidental, but is likely to be statistically significant as the probability of randomly matching a nonamer is almost negligible (1/(209)).
As expected, the obtained data suggested that all examined miRNAs shared a coincidental expression change between sRNA sequencing and RT-qPCR.
The probability of a coincidental occurrence of an amino acid sequence is P = Paa 1 × Paa 2×...Paa n.
We do not know whether the differential sRNA distribution within the hotspots may have any significance, although the highly coincidental profiles of the four independent sequencing experiments are intriguing.
While this makes for an arresting anecdote, the implicit assumption that the temporal sequence is anything other than coincidental is comprehensively debunked by the scientific and statistical evidence against it.
The probability of a coincidental occurrence of the nine amino acid sequence is 1.0 × 10-12 (see calculation in Methods) and we therefore suggest that the main part of oscillatorin is produced ribosomally as an integral part of this protein.
The essential challenge for in silico approaches is that the vast majority of motifs within the genome matching a particular transcription factor consensus sequence are likely to be nonfunctional, coincidental matches.
Some might say the timing of these two reports was purely coincidental; other cynical voices will wonder about possible sequencing.
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