Sentence examples for coin staining from inspiring English sources
We determined that the optimal concentration for COIN staining for further experiments is 0.25 mM.
We stained 2×106 cells in 200 µl COIN staining buffer with 0.1, 0.25, and 0.5 mM concentration of COINs.
We washed the cells in PBS (×1) and COIN staining buffer (×1) (PBST PBSS and 0.1% Tween 20)+10% fetal bovine serum (HyClone).
Thus, we have demonstrated the utility of COIN for staining cell lines as well as primary human samples.
To determine the utility of COINs for staining primary human cells, we stained human peripheral blood mononuclear cells (PBMC) with αCD8-conjugated COINs.
Before staining with COIN, we washed the cells in PBS and fixed them in 1.5% PFA for 15 minutes at RT.
We imaged the cells stained with COIN without additional processing, which is usually required for SEM, by using Quantomix capsules.
Fig. 4 a White light image of a formalin-fixed paraffin-embedded prostate tissue section stained with two COIN SEPs coded either with acridine orange (AOH) or basic fuchsin (BFU) and nucleic acid stain (YOYO).
The calculated changes in peak height ratio were statistically similar when we used two COINs simultaneously compared to using a single COIN in a staining assay.
We compared the Raman signals from cells stained with a single COIN to those of cells stained with both COINs.
The public soon complained that the new coins were becoming spotted and stained.
