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Most designed coiled coils function as oligomers, namely intermolecular coiled coils.
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VP35 mutants lacking the coiled-coil motif or possessing a mutation designed to disrupt coiled-coil function were defective in oligomerization, as deduced by co-immunoprecipitation studies.
For instance, p115 is a coiled-coil protein functioning as a Rab effector required for tethering of vesicles in RER-to-Golgi trafficking.
However, the major pitfall of SMASH is its high dependence on accurate coil sensitivity function, which might be easily affected by coil positions, subjects, and magnetic field inhomogeneities.
The sensor requires a single planar coil to function, which was constructed with conventional printed-circuit techniques along with the sensor signal conditioning circuitry.
This raises the possibility that flipping between these positions could allow the coiled-coil tail to function as a lever to exert force.
(B, C ) Average distance between the centers of the three α-helices at the N-terminus (B ) or the C-terminus (C ) of the coiled coil as a function of time in MD simulations.
However, the source coil and the device coil have the function of impedance matching which is achieved by adjusting the distance between the source coil or device coil and the resonators.
The use of coiled coils to mediate the binding of non-native proteins represents a novel strategy in chaperone design and a new function for coiled coils.
Yeast complex II contains instead another coiled coil protein, Vps38, and functions in endosome maturation.
One of the genes of interest is Pericentrin (PCNT), a gene which codes for a large coiled-coil protein with multiple functions in the cell.
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