In thyroid cells, the simultaneous expression of a cohort of tissue-specific transcription factors, including TTF1, TTF2, Pax8 and HEX has been described [ 19].
Tissue protein lysate arrays will help to automate and expand antibody-based validation of molecular targets in large cohorts of tissue specimens.
Less widely appreciated, is that TMAs also facilitate a rapid assessment of biomarker expression in larger cohorts of tissue and are thereby amenable to the development of statistical models based on structured immunohistochemistry information [ 8].
In addition, IHC staining of EMT markers (E-cadherin, α-catenin, vimentin and fibronectin) in our cohort of BC tissue microarray (TMA) tissues was utilised to analyse the EIF5A2 and EMT markers expression patterns in BC.
The training cohort consisted of tissue of 93 prostate cancer patients diagnosed between 1990 and 2001 at the Institute of Pathology, Charité - Universitätsmedizin Berlin.
A fourth sample cohort consisted of tissue samples from 21 necropsy cases of dogs whose clinical signs or microscopic lesions matched the sentinel animal (i.e., hemorrhagic diarrhea, vasculitis, and/or granulomatous disease); these samples were selected from the tissue archives of Anatomic Pathology at the UC Davis Veterinary Medical Teaching Hospital.
Most proteome studies use univariate analysis to compare the levels of a protein spot in a cohort of diseased tissue samples compared with normal tissues.
TMAs permit investigators to use a single slide to conduct controlled studies on large cohorts of tissues, using a small amount of reagent.
On the basis of miRNA microarray results, we experimentally verified expression levels of five miRNAs, miR-10b, miR-126, miR-196a, miR-204 and miR-215, in two independent cohorts of tissues with the 'gold-standard' qRT PCR using miRNA-specific TaqMan probes.
