Sentence examples for cohesion assays from inspiring English sources

For the mESC cohesion assays, 200 spreads were analyzed for each genotype.

Chromosome cohesion assay was performed as described previously [37].

Cohesion assay procedures were performed as previously described with the following modifications [39].

The resulting fragment was transformed into the CEN-proximal cohesion assay strain.

This cohesion assay strain also contains epitope-tagged Pds1p, allowing for detection of this inhibitor of anaphase onset [36].

To construct CEN-proximal cohesion assay strains containing ctf7eco1-1 ADE ctf7eco1-1 ADEining wild typlasmid was geneticontainingneered to contain ctf7eco1-1 DNA that harbors G211D.

Using a direct sister chromatid cohesion assay, we show that elg1 mutants indeed have a sister chromatid separation phenotype (Figure 5B), and have a 3.5-fold higher level of precocious separation, compared to wild type cells.

In order to directly measure this potential role we used a cohesion assay in which a TetO array is integrated approximately 40 kb from the centromere of chromosome V.

In addition, we found that ybp2Δ cells did not exhibit sister chromatid cohesion defects by the cohesion assay, using a strain an array of lactose operators integrated at the TRP1 locus, 12 kb from the CEN of chromosome IV and expressing a GFP-lactose repressor (GFP-lacI) [34], [35] (Supplemental Figure S4).

We extended the above analyses to include both wildtype and elg1 deletion mutant strains in the cohesion assay background and found that approximately 15% of large-budded elg1 mutant cells that retain Pds1p staining also contain separated sister chromatids (Figure 3).

To test this directly, wildtype, rad61 single mutant cells and rad61 elg1 double mutant cells were crossed into a cohesion assay strain in which TetO arrays are integrated approximately 40 kb from centromere V and detected via constitutive expression of GFP-tagged TetR-GFP TetO-binding protein.