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In addition to standard NCBI COGs [1] which include genes from at least three lineages, we also included genes present in fewer than 3 organisms.
We also use PSI-BLAST hits to COGs [8], [16].
A detailed classification using COGs [21] reveals other differences between CHR-O and N-CHR groups.
We compared the GOS clusters against the Clusters of Orthologous Groups (COGs) [34] using BLASTP.
Web-based software and databases were used to manually curate predicted genes after comparing data to public databases (COGs [16], Conserved Domains Database [17] and InterPro [18]).
These simulations were derived from diverse gene families that arise in genome-scale studies ("Collections of Orthologous Groups" or COGs, [20]).
Among these COGs, 4183 proteins were predicted as horizontally acquired.
Thus, only 2 COGs, CYP60B1-CYP65A2 pand and the CYP611A1-CYP636A1-CYP660A1 tripoor had poor bootstrap support.
Within this module two COGs, COG4624 (iron only hydrogenase) and COG3541 (predicted nucleotidyltransferase) were present.
As of October 2012, there were 5664 COGs, 1276 Bacterial and 114 Archaeal genomes sequences in the NCBI database.
In this way, we obtained 267 COGs with 0 gain and 0 loss events, 752 COGs with 0 gain and 1 loss, and 2,518 COGs with at least 1 gain and 1 loss event (Additional file 2, Figure S5).
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