In a holding pattern, too, are 350 pieces of what restaurateurs call holloware (tableside coffee servers, milk dispensers, teapots and ice buckets), some of which, like the bread stands, will be imprinted with the name.
If that's true, it's only because customers were tired of lazy coffee servers and craved the fast-food-like efficiencies of Starbucks.
All coffee servers know the terms small, medium, large, single, double and triple.
The sequences of AmNLG1, AmNLG3 and the mouse NLG1 sequence [51], were downloaded from Swiss Prot [95] (accession number Q99K10) and were aligned using the T-COFFEE server [96].
A multiple alignment of the remaining sequences was performed using the T-Coffee server [ 23].
All protein-coding genes were aligned separately using the T-Coffee server with the default parameter set [ 62].
> -wrap-foot> In practice, the most widely used public T-Coffee server is the one running on the Vital-IT platforms (http://tcoffee.vital-it.ch).
The quality of each resulting alignment was evaluated by the CORE method, available through the T-coffee server, compared and manually improved by removing any badly aligned columns.
A structure-based multiple sequence alignment was done with the T-Coffee server [ 53] and manually corrected on the basis of consensus secondary structure prediction [ 54].
Available c-type lectins and c1q sequences were retrieved from bivalve species present in Genbank, including Mytilus sp., Haliotis sp., Chlamys farreri, Mercenaria, and Ruditapes philippinarum and aminoacid protein sequences aligned using T-Coffee server [ 90], applying default settings.
Previously reviewed RAMP1 and RAMP3 full-length sequences were downloaded from the UniProt server, and a multiple sequence alignment was generated using the T-Coffee server (Poirot et al., 2003).
