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The low coefficients of variance of both the HBV and SIV standard stock results indicate the excellent reproducibility of the single-tube reagent.
A repeatable gas exchange process over 10 cycles was simulated with the coefficients of variance (COVs) of trapped mass and effective equivalence ratio being lower than 3%.
The low mean values of the coefficients of variance in repeatability in the QC-PCR method against the chitin assay demonstrated more reliability of this new approach.
The high coefficients of variance (CV > 50%; Table 2) and spatial distribution, illustrated by boxplots (Fig. 3), showed a heterogeneous abundance of most physicochemical parameters in dug wells.
The geometric means and the coefficients of variance were determined using WinMDI version 2.8 software.
The intra- and interassay coefficients of variance ranged from 8 15% and 5 16%, respectively.
We calculated intra and inter-assay coefficients of variance using 3 serum samples run in triplicate on three separate plates.
The minimum detectable level was 0.5 µg/dl, and interassay/intrassay coefficients of variance were 12.48% and 6.11%, respectively.
Inter- and intra-assay coefficients of variance were calculated in order to determine the accuracy of the assay.
The intra- and interassay coefficients of variance were determined by measuring five plasma samples in a total of six independent assays with duplicate determinations.
The intra-assay coefficients of variance (CVs) were 8 15% as evaluated by assaying 12 replicates of each sample in a single assay (Table 1).
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