The precision of the method was evaluated in terms of repeatability, obtaining a coefficient of variation of 2.54%.
Intra-subject coefficient of variation of AUC was 6.8%, and coefficient of repeatability was 11.8%.
Both repeatability of mean (R = 51.0%, F = 3.38, df = 73, 74, P < 0.0001) and that of coefficient of variation of population density (R = 65.5%, F = 4.80, df = 73, 74, P < 0.0001) estimated for all analyzed species were highly significant.
Repeatability was calculated from ten replicate determinations of a sample (in each case completely separate sample preparation) with a coefficient of variation of 2·4 % for the iso content and 0·06 % for the retention time was obtained.
The coefficients of variation for repeatability and reproducibility in assessments of anti-Xa activity were 1.0%and1.2%2%, respectively.
Finally, we used one-way ANOVAs to estimate repeatability of means and coefficients of variation of population size estimated for different species using the two groups of European regions.
The repeatability was studied at 20 μM providing coefficients of variation of 16% for AA and 13% for UA.
The repeatability of the proposed method was evaluated at two different spiking levels (10 and 50 µg L−1) obtaining coefficients of variation of 11.4% and 3.6% (n=3), respectively.
Measurement errors, expressed as coefficient of variation and the repeatability statistic, are provided in Table 1.
The coefficient of variation (CV) for intra-observer repeatability ranged from 2.0 2.2 % for both tibial and femoral cartilage volume measurements [ 16, 17].
