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The genes of most eukaryotic organisms, on the other hand, are separated by long stretches of intergenic DNA and their coding fragments, called exons, are interrupted by non-coding ones, called introns.
The neutral evolution of coding fragments (cal1 and las1) was tested by Tajima's test implemented in the same software.
Wild-type copies of the ctr4+ and ctr5+ genes were inserted downstream of and in-frame with the nonfluorescent C-terminal (VC) and N-terminal (VN) coding fragments of Venus, respectively.
In 3'RACE products from Pold1+/− ES cells, both full-length Pold1 coding fragments and truncated fragments were observed; while in the products from Pold1exo/+ ES cells, only the full-length fragment was observed.
The use of a sequence analysis tool (BESTORF, www.softberry.com) to predict potential coding fragments in these unannotated sequences, did not identify any open reading frames greater than 40 amino acids.
The neutral evolution of coding fragments (cal1 and chi18-5) was tested by Tajima test implemented in the same software.
Similar(41)
Genomic DNA from S. cerevisiae BY4741 was used as a template to amplify α-MF (Scα-MF) coding fragment.
Sequencing of the coding fragment of the M2 receptor gene revealed a single nucleotide mutation in 83% of hyperreactive animals, possibly contributing for the transcript overexpression.
This construct contained the same DLG-1 coding fragment that had previously been reported for the generation of rabbit and rat polyclonal antibodies [19].
cDNA for HA-RILP was generated by subcloned the coding fragment of RILP cDNA into pDHA vector.
We amplified 824 bp fragment of the SRY exon having 615 bp long coding fragment (Table 3B), cloned and sequenced.
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