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Because all sequences are protein coding, alignment could be done unambiguously by eye in a text editor.
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For the most conserved class of vertebrate alignments, 58% of non coding regions are alignable between human and fish, versus 95% of coding alignments (66.48% identity in alignable non coding sequences, 69.23% in alignable CDS).
We assessed the protein coding potential of these short transcripts using the QRNA software, which distinguishes between coding alignments, conserved non-coding alignments and RNA secondary structure [ 26].
In vertebrates, the top bin of non-coding sequences is substantially more conserved than coding in all mammals and chicken (mean Hs-Gg identity 84.63% for non-coding alignments, and 70.76% for coding alignments).
Figure 2 shows the distribution of GC% for the coding alignments.
After these quality filters, the coding alignments showed a mean nonsynonymous divergence of 2.29% (±0.02% 95 confidence interval [CI]) and synonymous site divergence of 10.44% (+0.05/−0.06% 95 CI).
These coding alignments showed a mean nonsynonymous divergence of 2.75% (±0.04% 95 CI) and synonymous site divergence of 11.1% (±0.13% 95 CI).
The rapid drop of the mean % identity of non coding alignments is due to the disappearance of CNC conservation in genome alignments of distant species, both for vertebrates and insects.
The last row compares it with another tool Target-align, which is available as standalone, serially coded alignment based tool.
Inspection of the protein-coding alignment for NA sequences showed no evidence of any of the known N2 neuraminidase inhibitor (NAI) resistance-associated mutations, R292K and E119V, in any of these Hong Kong influenza A(H3N2) isolates collected between 1997 2006.
For the chloroplast protein-coding alignment, five out of six comparisons had faster rates in the parasitic lineage.
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CEO of Professional Science Editing for Scientists @ prosciediting.com