Exact(4)
The fate of protease inhibitors released from genetically engineered plants is an important issue because of possible inhibition of soil proteases and effects of the insecticidal protein and its codifying sequence on soil microorganisms.
This oncogene consists of the NH2-terminal domain of FUS (previously termed translocated in liposarcoma, TLS) fused to the entire codifying sequence of DDIT3 (previously termed CHOP) [4], [5].
The coding sequence for full-length mouse Sall2 was synthetized by GeneScrip (http://www.genscript.com/) according to the Sall2 codifying sequence published in the Sanger database (http://www.sanger.ac.u) and was designed in fusion with the C-terminal GFP tag.
Also, it cannot be discarded that A. ferrooxidans DSM 16786 secretes a similar lipoprotein since the genome of strain ATCC 53993 possesses a codifying sequence for a protein with 96% amino acidic sequence identity.
Similar(56)
Different classes of organisms (e.g. prokaryotes, unicellular eukaryotes, and multicellular eukaryotes) as well as different genomes structures (e.g. codifying sequences, introns, and "junk" sequences) can present huge differences in constraints.
The finding of stop codons along with codifying sequences suggests the emergence of quasispecies, the presence of a high rate of viral evolution and thus the existence of defective interfering particles/heteroclites.
This sequence was used as template to clone SBM and RGD-SBM codifying sequences by PCR.
Even among codifying sequences, constraints act differently depending on the effect a possible mutation will generate on gene product.
To investigate three genes associated with puberty timing in girls with central precocious puberty by evaluating the association between polymorphism in the gene sequence codifying the enzymes participating in steroidogenesis, CYP1A1, CYP17, and CYP1B1 and central precocious puberty.
This effect could be partially explained by the ability to represses the transcription of t3766 (Fig. 4C), a sequence codifying a product with an unknown function that participates in the early survival of S. Typhi within monocytic cells (Fig. 3A).
Briefly, SBM and RGD-SBM sequences were cloned using the forward primers 5'-GGGAATT CCandTGACGTCAAACGTCACATTTAC-3' and 5'-GGGAATT CCATATG AGAGGTGATGGAGGCTCCGTTTCGATTTGG-3', respectively, and the reverse primer 5'-CCG CtheAGTGGCACATTCCAGCTCGC-3', the underline sequences are the restriction sites for the Nde I and Xho I, and the bold sequence codify the RGD.
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