Sentence examples for coded affinity from inspiring English sources

Isotope- coded affinity tag.

Data analysis from multiple platforms including high resolution mass spectrometry-based metabolomics, transcriptomics by RNA-sequencing, and redox proteomics by redox isotope coded affinity tag-based mass spectrometry was performed.

Briefly, peptides from particular samples are labeled with different tags, mixed and analyzed by MS. In metabolic labeling (e.g. SILAC) heavy isotope amino acids are incorporated during protein synthesis, while in chemical labeling (e.g. Isobaric tag for relative and absolute quantification (iTRAQ), Isotope- coded affinity tags (iCAT) are applied after tryptic digestion.

Introduction of quantitative proteomics approaches, such as isotope coded affinity tags (ICAT), allow obtaining more precise protein abundance estimates for a broader range of protein concentrations.

We used isotope coded affinity tagging and mass spectrometry to identify the scaffolding protein, receptor for activated C kinase (RACK1) as a protein enriched in M2-immunoprecipitates from M2-expressing cells over those of non-M2 expressing cells.

Protein expression was compared using the recently introduced cleavable isotope coded affinity tag (ICAT) methodology.

Chen et al. [ 6] used isotope-code affinity tag (ICAT) technology to compare the pancreatic juice protein profiling from pancreatitis patients and normal controls.

A novel class of isotope-coded affinity tag is proposed possessing a fluorescent feature, referred to as fluorescent isotope-coded affinity tag (FCAT), to provide a new tool for quantitative proteomics.

The chemically-coded affinity tag (CCAT) method combines standard electrophoresis protocols with MALDI-TOF-MS analysis to identify and quantify protein abundances in complex samples in one step.

The application of gel-free protein separation methods such as multidimensional protein identification technology (MudPIT), isotope-coded affinity tags (ICAT), and isobaric tags for relative and absolute quantification (iTRAQ) [43] began to be used widely along with the development of the mass spectrometer (MS).

To identify novel ubiquitination substrates of VHL, we undertook a proteomic screening using ICAT (isotope-coded affinity tag) quantitative proteomics technology [16], [25].