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Word processing software was used to code transcripts by themes.
Authors did not necessarily code transcripts in the same sequence.
We will draw on grounded theory and thematic analysis to code transcripts line by line.
We used qualitative analysis software (Atlas.ti) to code transcripts and facilitate analyses [ 29].
42 Three researchers will individually code transcripts from first-round interviews with patients as they are completed.
Trustworthiness was established through several aspects of the methodology, including having multiple researchers code transcripts and compare their findings; member-checking; and the selection of participants through purposeful sampling [ 30].
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Many of these are non-coding RNAs and shorter coding transcripts such as ribosomal proteins.
In addition to coding transcripts (mRNAs), non-coding microRNAs (miRNAs) appear to play a role in these processes.
Long non-coding RNAs (lncRNAs) are non-protein coding transcripts longer than 200 nucleotides.
Because novel protein-coding transcripts mingle with novel non-coding transcripts in the prediction process, we applied Coding Potential Calculator (CPC) [ 30] to evaluated protein-coding potential of novel transcripts and remove putative protein-coding transcripts.
Positive coding potential values reflect protein-coding transcripts whereas negative coding potential values designate non-protein-coding transcripts.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com