This mutant was isolated in a previous work, when a WT copy of the P110 and P140 coding genes was reintroduced in a class I mutant by transposon delivery [24].
A GFP coding sequence was then reintroduced by amplification from pEnT5-G with the primers (gc agatct GTGAGCAAGGGCGAGG and gc tctaga tta CTTGTACAGCTCGTCCATG), and insertion between the BglII and XbaI sites of the above plasmid.
We spent a month travelling through India just after its Supreme Court reintroduced the archaic Article 377 of the 1861 Penal Code.
To determine whether EEEV overwinters, is periodically reintroduced, or both, we sequenced the E2 and partial NSP3 coding regions of 42 EEEV isolates from New York State and the Eastern Seaboard of the United States.
The amended ordinance will be reintroduced in a meeting set for Feb. 9. Until then, city code allows cyclists to walk, or pedal, their bikes across the bridge.
Information about the sampled populations, year of sampling, sample codes, source of DNA, taxonomic status, geographical region of the sample localities, the indigenous or reintroduced status of populations and sample size.
To that end, we reintroduced a HindIII-modified IRES in the pPRIG vector, and cloned DsRed, which codes for a red fluorescent protein (RFP), under the direct control of the CMV promoter, creating the pPRIG-Hd-HA-Red.
When opposition to SB 561 surfaced, the bill's proponents probably fearing that they were sticking too closely to the historical script sanitized the language even further and reintroduced the bill as SB 733, the "Louisiana Science Education Act," this time emphasizing "critical thinking" as the operative code phrase (Louisiana Act 473 2008).
Warren reintroduced the bill.
How many were reintroduced?
