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This definition selected all cases of upper gastrointestinal bleeding that had either a supporting code (probable code) in the linked data or a less specific code (possible code) that did not contradict the bleeding diagnosis.
Therefore for each upper gastrointestinal bleed defined in either dataset from definition 1, a specific bleed code, probable code or possible code was searched for in the linked dataset within the 2 month time window and selected in the hierarchical order of the categories listed in Table 1.
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Based on ICPC codes and ATC codes probable chlamydia infections in 2007 were identified (similar to previous study) [ 13].
We assigned three stars to the evidence codes PROBABLE, EXP, IPI, IMP, IGI, IEP, ISS, ISO, ISA, ISM, IBA, IBD, IKR, IMR, IRD and IC.
'Possible' codes were defined as less specific codes that nevertheless indicated a non specific change in health state without indicating an alternative diagnosis to a gastrointestinal bleed (see Table 1 for categories of codes, 1=Most probable, 16=Less probable. The specific codes selected are listed in Additional file 1: Table S1 and Additional file 2: Table S2).
As a general rule, vital registration data, suitably corrected for ill-defined coding and probable systematic biases in certifying deaths to non-specific vascular, cancer and injury codes were used to estimate the cause of death pattern.
The corresponding polymorphisms were mapped to genes coding for probable porin gene (PA4137), probable ATP-binding component of ABC transporter (PA4909), rplA gene coding for 50S ribosomal protein (PA4273) and many conserved hypothetical protein ORFs namely PA0234, PA1370, PA1788, PA2228, PA3350, PA1091, and PA4203.
Interestingly, 4 genes encode putative proteins with significant similarity with transporters: 2 sucrose transporters (Ae_48AL7986 and Ae_29AL7339), a protein with an oligopeptide transporter domain (Ae_21AL7132; InterPro domain IPR004813), and a gene coding a probable mitochondrial substrate carrier (Ae_97AL5378).
To identify, via the SAIL Databank, all those with ICD-10 codes for probable and possible suicide in Wales 2003 2011, and matched controls.
These included genes with in-frame stop codons, deletion of conserved parts of the family protein sequence, frameshifts in coding exons, probable splice junction defects, and missing start codons.
In addition, gene PA3757 coding for probable trehalose operon repressor was significantly downregulated in the mutant strain (5.59-fold) which could result in observed increased expression of other trehalose metabolism-related genes.
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