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Full code for the downstream analysis is available upon request.
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To overcome this shortage, the SelGenAmic algorithm was developed to maximize the sensitivity in such way that an optimal i-ORF was built for each TGA codon, without skipping any TGA codon even with low coding bias for the downstream sequence.
The SelGenAmic algorithm we developed in this paper is to assemble i-ORF for each TGA in genomic sequences, while the GenAmic algorithm reported previously is to assemble selenoprotein genes and standard genes at the same time, using stronger coding bias for the sequence downstream Sec-TGA codon to discriminate real Sec-TGA codons and others.
If these step-related modulations are sufficiently robust to provide a code for downstream Purkinje cells, it should be possible to reconstruct the step sequence from the recorded activity.
Downstream, three putative genes appear to code for the components of an ABC transport system.
We used those sequences for the downstream in silico analyses.
Proper mixing takes significantly lower for the downstream sources model.
Among the genes coding for insulin signaling downstream of IRS1, significant up-regulation during the whole lactation was observed for AKT3 and PDPK1.
In order to obtain the chimeric construct, the gene fragment coding for the HP-RNase was cloned downstream to the Erb-hcAb sequence by inserting a spacer encoding an 11-amino-acid peptide linker (AAASGGPEGGS) between the scFv-Fc and the RNase.
The TSC genes, TSC1 and TSC2, code for proteins that act downstream the PI3K signaling pathway downregulating mTOR (Fig. 2).
As previously described [ 19], the Rasless cells were unable to proliferate, but did recover their proliferative ability after ectopic expression of transfected constructs coding for constitutively active downstream kinases of the Ras-MAPK pathway such as BRAFCAAX and MEK1Q56P.
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