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The cocoa samples were extracted and purified using molecular imprinted polymer (MIP) columns.
The developed aptasensor was extended to screen cocoa samples for OTA contamination.
The cocoa samples were extracted and purified using molecular imprinted polymer (MIP) columns specifically designed for OTA.
The system could detect OTA as low as 1.25 ng/ml with the maximum recovery of 87.5% in cocoa samples.
The present article describes a portable and low cost fluorescence set-up designed and characterized for in-situ screening of Ochratoxin A (OTA) in cocoa samples at field settings.
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Schlegel and Richter [29] analysed ten cocoa and chocolate samples and found between 50 and 150 mg kg-1 aluminium concentrations [29] which is within the same range as the values found in the present study.
Spence and his team asked the subjects of one study to liken both shapes and speech sounds (in the form of pseudo-words) to chocolate samples with varying cocoa content.
Regarding l-tryptophan, the highest content of this amino acid (13.27 13.34 μg g−1) was found in chocolate samples with the lowest cocoa content (70 85%).
This could be due to the differences in the sampling methods used (in that, cocoa bean samples for this study was taken from cocoa farms while samples used in the other studies were taken from cocoa bean samples ready for shipment in Ghana) or differences in the area of which the cocoa beans were coming from.
The composite cocoa bean samples were well mixed and 2 kg sub-samples were taken into clean labelled polythene bags and transported to the Ghana Standards Authority Pesticide Residue Laboratory in Accra, Ghana for analysis.
Ripe cocoa bean samples were taken from each farm, as well as a sample of cocoa beans that had been fermented and dried in their shells, ready for export.
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