Sentence examples for cocktail were added from inspiring English sources

After blocking the array in blocking buffer (PBS, 1% BSA, 0.1% Tween 20) for 1 h at 4°C, 10 µg aliquots of biotinylated NEMO or GST control diluted in 120 µl of probing buffer (PBS containing 0.5 mM DTT, 5 mM MgCl2, 5% glycerol, 0.05% Triton X-100 and Calbiochem protease inhibitor cocktail) were added to the array and the array covered and incubated at 4°C for a further 1.5 h.

AEBSF and the inhibitor cocktail were added right before use.

80ul of the detection antibody cocktail were added to each well, and incubated at room temperature for 2 h.

TdT enzyme and dUTP conjugated to a fluorescein cocktail were added to sections according to the manufacturer's specifications (in situ death detection kit; Roche Biochemical, Indianapolis, IN, USA).

At 96-h intervals additional volumes of the enzyme cocktail were added together with fresh biomass, at concentrations of 15 or 30 FPU per gram of glucan (7 or 14 mg enzyme protein per gram of glucan).

Next 500 μl of buffer 2 (20 mM Tris·HCl, pH 7.5, 0.5 M NaCl, 0.5% NP-40, 5 mM EDTA, 1 mM EGTA, 10 mM NEM, 1 mM DTT, 5 mM NaF, 1 mM Na3VO4 and protease inhibitor cocktail) were added, and the extracts were subjected to a 30-minute rotation at 4°C.

The resulting 100 μl of transfection cocktail was added to ARK2 cells pre-washed with OPTI-MEM.

To the supernatant, 70 ml of buffer II (pH 7.5) (20 mM potassium phosphate, 100 mM KCl, 24 mM imidazole and protease inhibitor cocktail) was added.

To this heady cocktail was added the architect Will Alsop, who, in 2006, teamed up with a firm called SMC, which, after some financial adventures, became Archial, which Alsop then announced he would leave in order to pursue his interest in painting.

Immediately thereafter, 15 ml of scintillation cocktail was added, the content was mixed and measured as above.

Protease inhibitor cocktail was added, after which the cell suspension was transferred to a pre-chilled tube with 0.5 mm glass beads and homogenized using a FastPrep®-24 (MP Biomedicals, Santa Ana, CA, USA) (4 cycles of 5.5 m/s for 30 s, 5 min resting on ice in between runs).