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From the analysis, an optimum ratio of cellulase cocktail was calculated by ANOVA of the response function as follows: Cellulase T Onozuka:Pectinase G = 0.333 0.388 0.286.
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The ratios of the numbers of IL-17-producing cells and proliferating CFSElow Th17 cells compared to those in the absence of the cytokine cocktail (considered as 1) was calculated for each response, and the fold changes are shown in Figure 4E 4F.
By immobilization of MBP-NcGRA2, MBP-NcSRS2, NcSAG1, separately, or the protein cocktail, ELISAs were carried out and the PP value for each sample was calculated.
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