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The denatured and prehybridised probe cocktail was applied onto the denatured chromosome preparation, overlaid with a coverslip and sealed with rubber cement.
The hybridization cocktail was applied to Human Genome HT_HG-U133_Plus_PM GeneChip 96-well arrays.
The antisense oligonucleotide/transfection reagent cocktail was applied to the cells and incubated for 7 h before the addition of 2 ml of culture medium with all supplements and 20% activated charcoal-stripped FBS.
After acetylation of the N-terminus with 10% acetic anhydride in DMF for 30 min, a cleavage cocktail was applied to the lanterns containing 95% trifluoroacetic acid (TFA), 2.5% H2O, and 2.5% triisopropylsilane (TIS) and incubated at RT for 2 h.
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Hybridization cocktails were applied to Affymetrix Rat Gene 2.0 microarrays and processed in accordance with the manufacturer's recommended procedure using the GCS3000 microarray system (Affymetrix).
Hybridisation cocktails were applied to Affymetrix Rat Gene 2.0 microarrays, and processed in accordance with the manufacturer's recommended procedure using the GCS3000 microarray system (Affymetrix).
In this study, an enzyme cocktail strategy was applied to choose several synergistic cellulases from different fungi and a designer concept was achieved for a cellulase cocktail production in a single host, using a synthetic biology technique.
A single-center, open-label, randomized, three-fold crossover, cocktail phenotyping design was applied.
For conventional single-condition maps, a cocktail-blank normalization procedure was applied as well as fixed clipping ranges for baseline, cooling, and recovery sets.
The h TERT/5q dual-colour FISH probe cocktail (Qbiogene, Montreal, QC, Canada) was applied at 0.02 0.06 μl mm−2 and sealed with rubber cement.
Cultures were lysed using BugBuster (EMD Millipore) supplemented with benzonase (EMD Millipore), a reducing agent, and the protein inhibitor cocktail, and the clarified lysate was applied directly to a prepacked column of Strep-Tactin Superflow (IBA Lifesciences).
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