Sentence examples for cocktail solution of from inspiring English sources

After removal of the solvent under vacuum, the residue was purified by silica gel chromatography (DCM MeOH = 1 20) to obtain coupling product in 90% yield, which was followed by treatment with a cocktail solution of TFA/DCM/triisopropylsilane (TIPSH) (95/2.5/2.5, 5 mL) to remove all of the protected groups after stirring at r.t. for 3 h.

The beads were then incubated with 100 µg of total cell lysate in 50 µl of kinase reaction buffer [10 µl of 5× kinase buffer and 10 µl of magnesium/ATP cocktail solution (90 µl of 75 mM MgCl2/500 mM ATP plus 10 µl (100 µCi) of [γ-32P]-ATP (3000 Ci/mmole)] for 30 min at 30°C.

Few cytotoxicity studies have investigated vitrification-relevant concentrations of CPAs, and the benefits and disadvantages of cocktail solutions and of incorporating non-permeating solutes have not been fully evaluated.

The culture was initiated by inoculation of 2.8% of culture volume into the prepared culture medium containing 50 μg/mL of kanamycin (Sigma, St . Louis MO, USA), 7.6 g/L of trace metal cocktail solution, 15.8 g/L of glycerol (Sigma, St . Louis MO, USA) and 1% (v/v) P2000 antifoam (Alfa Aesar, Reston, VA, USA) solution.

The selection of inhibitors and their concentrations for the preparation of inhibitor cocktail solution was based on the analysis of inhibitors composition in spruce hydrolysate used in this study (Table  1).

An aliquot (10 μl) of tissue extract was added to 200 μl of reaction cocktail solution (50 mM Tris HCl pH 8.0, 3 mM MgCl2, 3 mM DTT, 0.1 mM EGTA, 0.3 mM NADH, 2 mM fructose-6-phosphate, 10 IU aldolase, 80 IU triose phosphate isomerase, 14 IU glycerol-3-phosphodeshydrogenase, and 10 μl of distilled water).

After the optimum concentrations of MBP-NcGRA2, MBP-NcSRS2, and NcSAG1 had been decided, a cocktail solution containing all three kinds of protein at the appropriate concentration was made to act as the N. caninum detector.

Liver slices (0.5 g) were homogenized in 1.5 mL of buffer solution (50 mmol/l Tris, 150 mmol/l NaCl, and 10 ml/l Triton X-100, pH 7.2) [ 20] and mixed with 100 μl of proteinase inhibitor cocktail solution (P8340, Sigma-Aldrich, Inc., Saint Louis, USA).

The resin was suspended in the cocktail solution and the solution was shaken for 1 h.

Cells were harvested and lysed in RIPA buffer (GenDEPOT) with protease inhibitors (Xpert protease inhibitor cocktail solution, GenDEPOT) and phosphatase inhibitors (Xpert phosphatase inhibitor cocktail solution, GenDEPOT).

The inhibitor cocktail solution used in this study contained 12 different inhibitors (Table  1).